Comparison of McMaster and mini-FLOTAC fecal egg counting techniques in cattle and horses
Abstract
The
aim of this study was to compare two fecal egg count (FEC) techniques;
McMaster (McM) and Mini-FLOTAC (mF), for the detection of cattle and
horse gastrointestinal nematode eggs, in different locations. Experiment
1: feces were collected from 16 cattle and FEC was performed
individually, using mF with the sensitivity of 5 eggs per gram of feces
(EPG) and McM with a sensitivity of 50 EPG at Empresa de Pesquisa
Agropecuária de Minas Gerais - EPAMIG and the Laboratory of Parasitic
Diseases of the University of Parana – LDP/UFPR. Experiment 2: Fecal
samples from 30 horses were analyzed with mF (sensitivity of 5 EPG) and
McM (sensitivity of 25 EPG) at the University of Mato Grosso do Sul -
UFMS and LPD/UFPR. Experiment 3: feces were collected from 14 foals and
FEC was performed using mF (sensitivity of 5 EPG); and McM (sensitivity
of 25 EPG) only at the LPD/UFPR. For cattle, the average FEC of mF was
962 at LPD; and 1248 at EPAMIG; for McM it was 1393 at LPD and 1563 at
EPAMIG. For horses, the FEC average using the mF was 650 at LPD and 469
at UFMS; and for McM it was 677 at LPD and 554 at UFMS. For foals, the
average FEC for mF was 404 and 436 for McM. In all experiments, the
standard deviation and the coefficient of variation values were
significantly lower for mF. Therefore, it is recommended the use of the
Mini-FLOTAC technique, which is a method with less variability and
higher accuracy, particularly for animals with low FEC
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